Agrobacterium tumefaciens Mediated Genetic Transformation in Coker-312 (Gossypium hirsutum L.) Using Hypocotyls Explants
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چکیده
منابع مشابه
Development of efficient catharanthus roseus regeneration and transformation system using agrobacterium tumefaciens and hypocotyls as explants
BACKGROUND As a valuable medicinal plant, Madagascar periwinkle (Catharanthus roseus) produces many terpenoid indole alkaloids (TIAs), such as vindoline, ajamlicine, serpentine, catharanthine, vinblastine and vincristine et al. Some of them are important components of drugs treating cancer and hypertension. However, the yields of these TIAs are low in wild-type plants, and the total chemical sy...
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Cotton (Gossypium hirsutum L.) is an important fiber crop in Iran, cultivated on 150000-200000 ha of land. In Iran the estimated loss due to the insect pest is more than 30%. Traditionally, pests are controlled by 10-12 times spraying per growing season of environmentally harmful chemical insecticides (e.g. endosulfan and/or methosystox). In order to produce transgenic cotton resistance to in...
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Culture conditions were developed that induce Arabidopsis thaliana (L.) Heynh. root cuttings to regenerate shoots rapidly and at 100% efficiency. The shoots produce viable seeds in vitro or after rooting in soil. A transformation procedure for Arabidopsis root explants based on kanamycin selection was established. By using this regeneration procedure and an Agrobacterium tumor-inducing Ti plasm...
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E Keshamma, B. Madhusudhan, Department of Biochemistry, Center for Nano Science and Technology Research, Kuvenpu University, P.G. Centre, Shivagangotri Davangere 577 002, India; S. Rohini, M. Udaya Kumar*, Department of Crop Physiology, University of Agricultural Sciences, GKVK Bangalore 560 065, India; K. S. Rao, Centre for Ecological Sciences, Indian Institute of Science, Bangalore 560012, In...
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A rapid Agrobacterium tumefaciens-mediated transformation system for wheat was developed using freshly isolated immature embryos, precultured immature embryos, and embryogenic calli as explants. The explants were inoculated with a disarmed A. tumefaciens strain C58 (ABI) harboring the binary vector pMON18365 containing the [beta]-glucuronidase gene with an intron, and a selectable marker, the n...
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2017
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2017.612.321